Assessment of Genetic Variation Among Leptosphaeria maculans Isolates Using Pathogenicity Data and AFLP Analysis

Analysis of two pathogenicity genes of the blackleg fungus Leptosphaeria maculans

Rapid identification of genetic variation and pathotype of Leptosphaeria maculans by random amplified polymorphic DNA assay.

Canadian isolates of Leptosphaeria maculans, the causal agent of blackleg of crucifers, were examined for genetic relatedness by the random amplified polymorphic DNA assay. DNA polymorphisms amplified with random decamer primers were used to distinguish three groups of isolates. Group 1 contained all isolates of the virulent pathotype, group 2 contained isolates of the avirulent pathotype from ...

Epidemiology / Épidémiologie Prevalence of pathogenicity groups of Leptosphaeria maculans in western Canada and North Dakota, USA

Blackleg, caused by Leptosphaeria maculans, is an economically important disease of canola (Brassica napus). Little is known about the current distribution of pathogenicity groups (PGs) of L. maculans in Canada and the United States. Four hundred and eighty-nine isolates of L. maculans or Leptosphaeria biglobosa from western Canada and North Dakota, United States, were placed in five PGs (PG-1 ...

Genetic variation among Escherichia coli isolates from human and calves by using RAPD PCR

Background: Various strains of Escherichia coli (E. coli) are known as major causes of intestinal and extraintestinal infections in humans and various animal species. Molecular methods are important for the identification of bacterial isolates and nucleotide sequence variations, as well as information on tracking bacterial agents related to the outbreaks, the frequency of the bacterial genetic ...

Assessment of Genetic Structure and Variation of Native Berberis Populations of Khorasan Provinces (Iran) Using AFLP Markers Versus Morphological Markers

Seedless barberry (Berberis vulgaris L. var. asperma) is one of the few crops that is only cultivated in eastern parts of Iran. As a new crop there has not been any study to identify phylogenetic relationships of this plant with other related species existing in Iran. In this study, Amplification fragment length polymorphism (AFLP) markers based on four selected primer combinations (EcoRI/Tru1I...